NF ISO 22942-1

NF ISO 22942-1

May 2022
Standard Current

Molecular biomarker analysis - Isothermal polymerase chain reaction (isoPCR) methods - Part 1 : general requirements

This document specifies general criteria for development, validation and use of nucleic acid analytical methods based on the isothermal polymerase chain reaction (isoPCR). It provides additional information and guidance for specific isoPCR technologies. This document is applicable to food, feed, plant matrices and their propagules, plant pathogens, and animals in which amplification of a specific biomolecular target sequence is required.

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Main informations

Collections

National standards and national normative documents

Publication date

May 2022

Number of pages

46 p.

Reference

NF ISO 22942-1

ICS Codes

67.050   General methods of tests and analysis for food products

Classification index

V03-511-1

Print number

1

International kinship

ISO 22942-1:2022
Sumary
Molecular biomarker analysis - Isothermal polymerase chain reaction (isoPCR) methods - Part 1 : general requirements

This document specifies general criteria for development, validation and use of nucleic acid analytical methods based on the isothermal polymerase chain reaction (isoPCR). It provides additional information and guidance for specific isoPCR technologies.

This document is applicable to food, feed, plant matrices and their propagules, plant pathogens, and animals in which amplification of a specific biomolecular target sequence is required.

Table of contents
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  • Avant-propos
  • 1 Domaine d'application
  • 2 Références normatives
  • 3 Termes et définitions
  • 4 Principe
  • 5 Mise au point d'une méthode isoPCR
  • 6 Exigences générales du laboratoire et du mode opératoire
  • 7 Matériaux et réactifs
  • 8 Interprétation des résultats
  • 9 Rapport d'essai
  • Annexe B (normative) Utilisation de témoins
  • Annexe A (informative) Informations minimales à fournir pour une expérience isoPCR (MIIPCRE)
  • Annexe C (informative) Exemples de résultats d'amplification isotherme isoPCR d'acide nucléique
  • Annexe D (informative) LAMP (loop mediated isothermal amplification)
  • Annexe E (informative) RCA (rolling circle amplification)
  • Annexe F (informative) HDA (helicase dependent amplification)
  • Annexe G (informative) RPA (recombinase polymerase amplification)
  • Annexe H (informative) SDA (strand displacement amplification)
  • Annexe I (informative) NASBA (nucleic acid sequence based amplification)
  • Annexe J (informative) Amplification Cas9nAR
  • Bibliographie
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