NF EN 12955

NF EN 12955

October 1999
Standard Cancelled

Foodstuffs - Determination of aflatoxin b1 , and the sum of aflatoxins B1, B2, G1 and G2 in cereals, shell-fruits and derived products - High performance liquid chromatographic method with post column derivatization and immunoaffinity column clean up

Le présent document spécifie une méthode de détermination des teneurs en aflatoxi-nes supérieures à 8 microgrammes/kg dans des aliments tels que maïs, beurre d'arachide,céréales, produits oléagineux, fruits secs,...

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Main informations

Collections

National standards and national normative documents

Publication date

October 1999

Number of pages

17 p.

Reference

NF EN 12955

ICS Codes

67.060   Cereals, pulses and derived products
67.080.10   Fruits and derived products
67.200.20   Oilseeds

Classification index

V03-122

Print number

1 - 26/11/2004

European kinship

EN 12955:1999
Sumary
Foodstuffs - Determination of aflatoxin b1 , and the sum of aflatoxins B1, B2, G1 and G2 in cereals, shell-fruits and derived products - High performance liquid chromatographic method with post column derivatization and immunoaffinity column clean up

Le présent document spécifie une méthode de détermination des teneurs en aflatoxi-nes supérieures à 8 microgrammes/kg dans des aliments tels que maïs, beurre d'arachide,céréales, produits oléagineux, fruits secs,...
Standard replaced by (1)
NF EN ISO 16050
October 2011
Standard Current
Foodstuffs - Determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products - High-performance liquid chromatographic method

<p>ISO 16050:2003 specifies a reverse-phase high-performance liquid chromatographic method, with immunoaffinity column clean-up and post-column derivatization, for the determination of aflatoxins in cereals, nuts and derived products. The limit of quantification for aflatoxin B<sub>1</sub>, and for the sum of aflatoxins B<sub>1</sub>, B<sub>2</sub>, G<sub>1</sub> and G<sub>2</sub>, is 8 micrograms per kilogram.</p> <p>The method has been validated for maize containing 24,5 micrograms per kilogram, for peanut butter containing 8,4 micrograms per kilogram, and for raw peanuts containing 16 micrograms per kilogram of total aflatoxins. It has also been shown that this method can be used for oilseed products, dried fruits and derived products.</p>

Table of contents
  • Avant-propos
    3
  • 1 Domaine d'application
    4
  • 2 Références normatives
    4
  • 3 Principe
    4
  • 4 Réactifs
    4
  • 5 Appareillage
    7
  • 6 Mode opératoire
    8
  • 7 Calcul des résultats
    9
  • 8 Fidélité
    10
  • 9 Rapport d'essai
    11
  • Annexe A (informative) Données relatives à la fidélité
    12
  • Annexe B (informative) Bibliographie
    14
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